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Mohamed Rashwan
2. Materials and methods

This experiment was carried out at Al-Hamra Village, Wadi Al

Natrun area, El-behera Governorate, during the optimum condition
of maize cultivation ( form May to November in the years of 2014

and 2015).

A field experiment was divided into four equal homogenous lon-
gitudinal areas. The main area was divided into four replicates. These

plots were separated from each other by proof tracks (1.5 m width)
to avoid leakage of different concentration levels of foliar proline.

The four replicates were designed for each treatment. Each plot has
a length of 10 meters and a width of 3.5 m. The whole experiment

was divided into five lines with 70 cm a part end and the distance
between successive plants is 30 cm. At the beginning, maize seeds

were separated into four parts (T0, T1, T2 and T3) and mashing the
seeds (T1, T2 and T3) for two hours in different concentration solu-

tions of proilne (15, 30 and 45ppm), respectively. Then, three seeds
were placed in each of the holes and reduced to one after germina-

tion. Proline was sprayed once a week during plant growth according
to the designed treatments.

2.1. Water, soil and plant sampling:

Water samples collection and analyses were carried out accord-

ing to standard methods for examination of water and waste water
(APHA, 2012). Three soil samples were collected at the depths of 0 –

20, 20 – 40 and 40 – 60 cm and kept in polyethylene bags before be-
ing sealed by twisting and tying the neck and dried, crushed to finely

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